There is my list with 7 best PyCon 2016 videos. I don't say that other videos not good. I say that these videos are more useful for me or impress me more than others. And as you can see I prefer talks about Python internals. I hope you'll find this list helpful.
Larry Hastings - Removing Python's GIL: The Gilectomy
Andrey Petrov - See Python, See Python Go, Go Python Go
Chelsea Voss - Oneliner-izer: An Exercise in Constrained Coding
Russell Keith-Magee - A tale of two cellphones
Glyph - Shipping Software To Users With Python
Scott Sanderson, Joe Jevnik - Playing with Python Bytecode